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KMID : 1007520150240051893
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Food Science and Biotechnology
2015 Volume.24 No. 5 p.1893 ~ p.1896
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BEFS-PPARγ2 Cells Incubated with trans-11 C18:1 Exhibit More Beneficial Fatty Acid Synthesis
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Wang Tao
Lee Hong-Gu
Wu LiFang
Qin GuiXin
Lou YuJie
Sun ZeWei
Guo Wei
Yang Jian
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Abstract
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Responses of the PPARγ2 (an adipogenic lineage determinant) over-expressed immortalized bovine embryonic fibroblast cell line (BEFS-PPARγ2 cells) to trans-11 C18:1 (TVA) incubation were evaluated. BEFS-PPARγ2 cells were well differentiated. Stearoyl-CoA desaturase 1, an endoplasmic reticulum enzyme that can catalyze conversion of TVA into cis-9, trans-11 CLA, exhibited a significantly (p<0.05) higher mRNA expression after 4 h, compared with controls. Enzyme activity peaked, and was significantly (p<0.05) higher after 6 h, compared with controls. With extension of the incubation time, TVA absorption into cells was significantly (p<0.05) higher with significantly (p<0.05) more conversion into cis-9, trans-11 CLA, compared with shorter incubation times. TVA incubated cells exhibited significantly (p<0.05) higher levels of arachidonic, eicosapentaenoic, and docosahexaenoic acids than controls. BEFS-PPARγ2 cells can be used as a model system and TVA incubation is a good approach for research into cis-9, trans-11 CLA-fortified beef production.
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KEYWORD
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BEFS-PPARγ2 cell, trans-11 C18:1, cis-9, trans-11 CLA
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